TABLE 1.
Primer-probe sets for 5′ nuclease real-time RT-PCR
| Set no. | Targetb | Sequencea
|
||
|---|---|---|---|---|
| Forward primer | Probe | Reverse primer | ||
| 1 | E | TCAGCGATCTCTCCACCAAAG (1160V) | TGCCCGACCATGGGAGAAGCTC (1186V) | GGGTCAGCACGTTTGTCATTG (1229C) |
| 2 | NS1 | GGCAGTTCTGGGTGAAGTCAA (3111V) | TGTACGTGGCCTGAGACGCATACCTTGT (3136V) | CTCCGATTGTGATTGCTTCGT (3239C) |
| 3 | 3′ UTR | CAGACCACGCTACGGCG (10668V) | TCTGCGGAGAGTGCAGTCTGCGAT (10691V) | CTAGGGCCGCGTGGG (10770C) |
a
Designations in parentheses are nucleotide number and polarity of the primer-probe. V, viral genomic sense; C, complementary sense. Nucleotide numbering is based on the sequence from Lanciotti et al. (14; GeneBank accession number AF196835).
b
The sequences for the primer-probe sets were designed by R. S. Lanciotti, CDC (14; Lanciotti, personal communication).