TABLE 2.

Comparison of RNA extraction by ABI Prism 6700 workstation and with RNeasy^a

No. of PFU spiked	CT value
	Kidney		Heart		Brain		Spleen
	6700	RNeasy	6700	RNeasy	6700	RNeasy	6700	RNeasy
103	21.4	21.44	21.36	21.4	20.74	20.75	21.39	21.29
102	24.7	24.89	24.89	25	24.85	24.88	24.77	24.8
10	28.22	28.13	28.22	28.15	28.14	28.19	28.18	28.18
1	31.14	31.48	31.4	31.52	30.59	31.37	31.38	31.34
10−1	34.86	35.23	34.89	35.18	35.73	34.32	34.84	34.66
10−2	36.93	37.08	37.46	36.28	36.96	38.81	37.98	37.67
10−3	40	40	40	40	40	40	40	40
0	40	40	40	40	40	40	40	40

^aKidney, heart, brain, and spleen from an uninfected bird were spiked with a known amount of WNV, and the homogenized samples were subjected to RNA extraction by the RNeasy method and with the ABI Prism 6700 workstation (6700). The recovered RNA samples were analyzed by real-time RT-PCR. See details in Materials and Methods.