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. Author manuscript; available in PMC: 2023 Feb 15.
Published in final edited form as: Arch Biochem Biophys. 2021 Dec 20;716:109111. doi: 10.1016/j.abb.2021.109111

Figure 1. Genotyping and western blot results of the ApoA-I−/−/Bco2−/− mice and the Bco2−/− mice.

Figure 1.

(A) Genotyping results of mutated (MT) and wild type (WT) ApoA-I genes of the mouse by PCR. The PCR products of mutated and wild type Apo A-I genes are ~ 250 bp and 169 bp, respectively. (B) Genotyping results of MT and WT mouse Bco2 genes by PCR. The PCR product of the mutated Bco2 gene is ~790 bp, while that of the wild type gene is ~212 bp. (C) Western blot. A ~25Kd protein band was detected in the Bco2−/− mice but not in the ApoA-I−/−/Bco2−/− mice. The data of PCR and western blot demonstrated that the ApoA-I−/−/Bco2−/− mouse line had been successfully generated.