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. Author manuscript; available in PMC: 2023 Jan 24.
Published in final edited form as: Curr Biol. 2021 Nov 17;32(2):289–303.e6. doi: 10.1016/j.cub.2021.10.063

Figure 5 |. Derived Shox2 expression in jerboa metatarsals may have contributed to increased distal limb elongation.

Figure 5 |

(A-B) Shox2 is expressed in the proliferative zone (PZ) of jerboa metatarsals (MT) where it is not present in mouse. (C) Open chromatin regions in P5 jerboa and mouse growth cartilage near the Shox2 transcription start site (TSS, green arrow). A 139 bp region of open chromatin, preferentially accessible in jerboa metatarsals (orange), lies ~285 kb upstream of the Shox2 TSS in an Rsrc1 intron at a position orthologous to mouse chr3:67266489–67267716. (D,F) Metacarpal and (E,G) metatarsal lengths are increased in Prx1Cre;LSL-Shox2 (Shox2-overexpressing) mice at eight weeks of age compared to control littermates. In (H), measurements are normalized to the skull length of each individual, which was not affected by Shox2-overexpression (Figure S6D). p-values are derived from a paired t-test between matched-sex littermates (n=5 females and 2 males of each genotype). Shox2-overexpressing metacarpals are 23.6% and metatarsals are 9.1% longer than controls. Scale bars, (A-B) = 50 μm, (D-G) = 2 mm. See also Figure S6.