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Rutin prevented the Ero1α/PDI-catalyzed increase of reduction potential of plasma glutathione.
| GStotal (μM) | GSH (μM) | GSSG (μM) | GSH/GSSG | E’ (mV) | |
|---|---|---|---|---|---|
| Buffer | 26.8 ± 2.2 | 23.8 ± 2.1 | 3.0 ± 0.4 | 8.2 ± 1.1 | −148 ± 3 |
| DMSO + PDI + Ero1α | 26.8 ± 2.6 | 23.1 ± 2.3 | 3.7 ± 0.3 | 5.2 ± 0.3 | −140 ± 1* |
| Rutin + PDI + Ero1α | 27.0 ± 3.0 | 24.0 ± 1.8 | 3.0 ± 0.4 | 7.8 ± 0.8 | −149 ± 2 |
The reduction potential was calculated as described in Table 1. *P < 0.05, compared to buffer. Unpaired Student's t-test.
