TABLE 1.

Histopathological and bacteriological status of tissue specimens and performance of HBTC-PCR and LC-PCR for detecting M. bovis in tissue

Culture result	Specimen no.	Skin test result	Histopathology	Days to positive BACTEC culture	HBTC-PCR resultd	LC-PCR result
Positive	2	+	+	14	−	−
	3	+	+	14	+++	−
	4	+	+	14	++	−
	5	+	+	14	+++	+
	6	N/Aa	+	14	+	+
	8	N/A	+	7	+++	+
	9	N/A	+	14	+++	+
	12	N/A	+	14	+++	+
	19	+	+	14	+++	+
	20	+	−	14	+++	+
	22	+	+	14	+++	+
	23	N/A	+	14	+	+
	24	N/A	+	14	+++	+
	25	N/A	+	14	+	−
	26	N/A	+	14	+++	+
	31	N/A	−	14	++	+
	32	N/A	+	7	+	−
	33	+	+	14	+	+
	36	N/A	+	14	+++	+
	38	N/A	+	14	+	−
	39	N/A	+	7	+++	+
	41	N/A	+	14	−	−
	42	+	−	14	++	+
	43	+	−b	—c	+++	+
	44	+	−	7	++	+
	46	N/A	+	14	+++	+
	49	N/A	+	14	+	−
	50	N/A	+	14	++	+
Negative	1	+	−		−	−
	7	N/A	+		−	−
	18	N/A	+		−	−
	21	N/A	−b		−	−
	27	N/A	−b		−	−
	28	N/A	−b		−	−
	29	N/A	−b		−	−
	34	+	−		−	−
	47	N/A	−b		−	−
	48	N/A	+		−	−

^aN/A, animals were not skin tested prior to analysis. 

^bActinomyces infection. 

^cA few colonies grew on L-J slope media. 

^d+++, strong band; ++, medium band; +, weak band.