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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Trauma Acute Care Surg. 2022 Feb 1;92(2):330–338. doi: 10.1097/TA.0000000000003461

Figure 3. Effect of trauma plasma on receptor-independent total versus intracellular PMN RB.

Figure 3.

Healthy PMN were incubated with 10% biofluids for 25 min and then PMA-induced RB was measured for 60 min using a luminometer (left hand traces). AUCs were calculated for 60 min (right hand graphs) due to the slower onset of PMA activation. We noted that total RB (panel A) was equally and markedly suppressed by trauma plasmas from Day 0–1 and Day 2–3. Intracellular RB (panel B) is not significantly suppressed. Note that intracellular RB (B) peaks later than total RB (as seen in panel A or in Fig. 2) and is generally much greater than receptor-initiated RB. AUC results were compared using one-way ANOVA / Tukey’s test (**p<0.01). CP, control plasma; TP, trauma plasma; RLU, relative light units.