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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Trauma Acute Care Surg. 2022 Feb 1;92(2):330–338. doi: 10.1097/TA.0000000000003461

Figure 5. Effect of trauma biofluids on PMN chemotaxis and NETosis.

Figure 5.

Healthy PMN were incubated with 10% biofluids for 25 min. In Panel A, PMN were pre-incubated in 10% volunteer control plasma, wound fluid or trauma plasma collected from the same patient at the same time as the wound fluid. CTX towards fMLF (100 nM) was then studied in transwells. Migrated PMN were retrieved after 60 min and CyQuant dye was used to quantify cell numbers. Chemokinesis (cells spontaneously migrating without fMLF being present) was subtracted. Both plasma and wound fluids markedly suppress chemotaxis. In Panel B, PMN were pre-incubated in buffer (‘None’) 10% volunteer (Control) or trauma plasma (Trauma). Extracellular trap formation (NETosis) was then induced by PMA over 4 hours and then measured using the elastase technique. Trauma plasma markedly suppressed NETosis as compared to volunteer plasma. Values were compared using one-way ANOVA / Tukey’s test (*p<0.05; **p<0.01; ***p<0.005).