Table 3: Maximum incubation time (3 days) between blood collection in EDTA tubes and initial processing prior to loss of common T and B cell surface markers.

For baseline analysis (left side), a range of −20% to +20% of the Day 1 (D1) value for all samples and populations was used for quantitative analysis. Subsequent timepoint population values were examined and flagged if out of range. The out-of-range rate was calculated at each timepoint by the number of flagged populations out of the 20 total parameters examined per timepoint (5 populations for each of the 4 samples per timepoint). Total number of flags and out of D1 range rates are shown on the left. For inter-assay precision analysis (right side), the %CV for each population at each timepoint in comparison to the D1 value was determined. Values were flagged if out of range. Total number of flags and out of inter-assay range rates are shown on the right.

	Parameters Out of D1 Baseline Range				Parameters Out of D1 Inter-Assay Precision Range
Post-Collection Timepoint	D2	D3	D4	D7	D2	D3	D4	D7
CD3+	0	0	1	2	0	0	1	2
CD4+	0	0	0	1	0	0	2	2
CD8+	0	1	2	1	0	2	2	2
CD3−	1	0	3	4	0	0	1	3
CD19+CD22+	2	2	3	3	2	2	3	3
Total Number of Flags	3	3	9	14	2	4	9	12
Out-of-Range Rate (%)	15	15	45	55	10	20	45	60