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. Author manuscript; available in PMC: 2023 Jan 24.
Published in final edited form as: Curr Biol. 2021 Dec 6;32(2):386–397.e6. doi: 10.1016/j.cub.2021.11.032

Figure 2. RNAi screen identifies PATR-1 as an essential component of progenitor cell P-bodies.

Figure 2.

(A) Progenitor cells stained for TRAL (red), progenitor cells (green), and DAPI (blue) after knockdown of selected genes. (B) Numbers per phenotypic class. (C) PMG stained for PROS (red), PATR-1 (green), HRP (white) and DAPI (blue). C’ and C” are enlargements of indicated regions. (D-G) Progenitor cells from control and Patr-1 RNAi intestines stained for PATR-1, EDC3, GE-1, or Me31B. (H-K) Super-resolution micrographs of control and Patr-1 RNAi intestinal progenitors stained for Me31B (green), DAPI (blue) and either TRAL or EDC3 (red). (L-O) Normalized pixel-by-pixel fluorescent intensity profiles of indicated protein (TRAL, Me31B or EDC3) along white lines in H-K. Full genotypes listed in Data S1F. See also Figure S2.