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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2021 Dec 2;42(2):113–126. doi: 10.1161/ATVBAHA.121.316090

Figure 3: Macrophage-specific IGF-1 overexpression changed atherosclerotic plaque toward a more stable plaque phenotype.

Figure 3:

A, E Serial aortic valve cross-sections were stained with Mac3 antibody, (N=16–29 mice per group). B, G. Aortic valve section were stained with trichrome (N=16–29 mice per group). C, H. Snap-frozen aortic valve sections were stained with Oil Red O (N=6–7 mice per group). D, I. TUNEL assay co-stained with Mac3 antibody (N=5–6 mice per group). Data is normalized to 1000 Mac3+ cells. Insert showing TUNEL+/Mac3+-positive plaque cells. F. Mac3/DAPI double positive cell number was normalized to total number of DAPI positive cells in the plaque. (N=13 mice per group). Scale bar, 100 μm. All statistical tests are Student’s two-tailed t-test, except (G), which has a Welch’s correction to account for a difference in SDs. L=Lumen