Skip to main content
. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2021 Dec 2;42(2):113–126. doi: 10.1161/ATVBAHA.121.316090

Figure 4: Macrophage-specific IGF-1 overexpression reduced monocyte recruitment into atherosclerotic plaque and decreased CXCL12 chemokine expression.

Figure 4:

A, B. Monocyte recruitment was measured by quantification of plaque levels of red beads after normalization to plaque size. Arrows, plaque red spots (N=4–7 mice per group). C. Circulating levels of CXCL12 measured by ELISA (N=8–11 mice per group). D, F. CXCL12 positive area was normalized to plaque area (N=10 mice per group). E,G. CXCL12/Mac3 positive area was normalized to cell number (N=8–10 mice per group). H. CXCL12 mRNA levels in LCM plaque isolates (N=3–5 mice per group). I. CXCL12 levels in peritoneal macrophages were quantified by immunoblotting. Predicted weight 7–14kDa. (N=8–9 mice per group). Scale bar, 100 μm. All statistical tests are Student’s two-tailed t-test, except B, which had a Welch’s correction due to differences in SDs. AP=Atherosclerotic Plaque, L=lumen