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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Cell Chem Biol. 2021 Nov 22;29(1):157–170.e6. doi: 10.1016/j.chembiol.2021.11.001

Figure 1. Targeted laser pulses induce localized lipid peroxidation in live cells.

Figure 1.

A. Schematic diagram describing the procedures of the photochemical activation of membrane lipid peroxidation (PALPv1) technique. BODIPY-C11 at a concentration of 5 μM was added to the cells approximately 30 min prior to imaging. Then a region in the cell was targeted by 5 pulses of the 405 nm laser. Each pulse lasts for 1 second. The 488 nm channel intensity was then recorded for 25 seconds at 1-second intervals to monitor the oxidized BODIPY-C11 signal.

B. Representative fluorescent images showing the time course of the reduced and oxidized BODIPY-C11 signals before and after the application of 405 nm laser pulses in wildtype 786-O cells. Scale bar indicates 10 μm. Five independent experiments were performed.

C. Representative fluorescent images showing the reduced and oxidized BODIPY-C11 signal before and after the application of 405 nm laser pulses in primary cortical neurons after 2-day culture in vitro. Scale bar indicates 10 μm. This experiment was performed once and 9 neurons were analyzed.