| Antibodies |
| Anti-FACL4 antibody |
Abcam |
Cat# ab155282, RRID: AB_2714020 |
| Anti-AGPS |
Abcam |
Cat# ab236621 |
|
β Actin Mouse mAb |
Cell Signaling Technologies |
Cat# MA5–11116, RRID: AB_10985704 |
| IRDye 800CW goat-anti-Rabbit secondary antibodies |
LiCor |
Cat# 925–32211, RRID: AB_2651127 |
| IRDye 680RD donkey-anti-Mouse secondary antibodies |
LiCor |
Cat# 925–68072, RRID: AB_2814912 |
| Anti-Cytochrome P450 Reductase antibody |
Abcam |
Cat# ab180597 |
| Alexa Fluor 647 anti-rabbit secondary antibodies |
Beyotime |
Cat# P0180 |
| Chemicals, peptides, and recombinant proteins |
| penicillin/streptomycin |
Gibco |
Cat# 15140–122 |
| GlutMax |
Gibco |
Cat# 35050061 |
| B27 |
Gibco |
Cat# 17504001 |
| ML210 |
Cayman |
Item No. 23282 |
| 1S,3R-RSL3 |
Cayman |
Item No. 19288 |
| ferrostatin-1 |
Cayman |
Item No. 17729 |
| liproxstatin-1 |
Cayman |
Item No. 17730 |
| deferoxamine |
Cayman |
Item No. 14595 |
| BODIPY-C11 |
Life Technologies |
Cat# D3861 |
| DMSO |
Sigma-Aldrich |
Cat# D2650 |
| Oleic acid |
Cayman |
Item No. 90260 |
| arachidonic acid |
Cayman |
Item No. 10007268 |
| docosapentaenoic acid |
Cayman |
Item No. 90165 |
| docosahexaenoic acid |
Cayman |
Item No. 17950 |
| BSA |
Sigma-Aldrich |
Item No. 10010566 |
| puromycin |
Gibco |
Cat# A11138–03 |
| Lipofectamine™ 2000 Transfection Reagent |
Thermo Fisher |
Cat# 11668019 |
| polybrene |
Sigma-Aldrich |
Cat# TR-1003-G |
| lithium dodecyl sulfate sample buffer |
Novus |
Cat# NP0008 |
| isopropanol, HPLC Grade |
Thermo Fisher |
CAS: 67–63-0 |
| ammonium acetate |
Sigma-Aldrich |
Cat# 73594 |
| methanol |
Sigma-Aldrich |
Cat# 34885 |
| formic acid, HPLC LiiChropur |
Supelco |
Cat# 5.33002 |
| 2,5-dihydroxybenzoic acid |
Sigma-Aldrich |
Cat# 39319 |
| trifluoroacetic acid |
Sigma-Aldrich |
Cat# 80457 |
| arachidonic acid-alkyne |
Cayman |
Item No. 10538 |
| Critical commercial assays |
| Lenti-X Go-Stix Plus |
TakaraBio |
Cat# 631280 |
| CellTiter-Glo Luminescence Assay |
Promega |
Cat# G7571 |
| BCA Protein Assay kit |
Pierce |
Cat# 23225 |
| Click-iT® Lipid Peroxidation Detection with Linoleamide Alkyne |
Invitrogen |
Cat# C10446
|
| Deposited data |
| Lipidomics data of ACSL4-depleted 786-O cell |
This paper |
Supplementary Table1
|
| Lipidomics data of ES-2, OVCAR8, SKOV3 and OV56 cell lines |
This paper |
Supplementary Table2
|
| Experimental models: Cell lines |
| Human clear-cell renal cell carcinoma cell: 786-O |
ATCC |
Cat# CRL-1932, RRID: CVCL_1051 |
| Human clear-cell ovarian carcinoma cell: ES-2 |
ATCC |
Cat# CRL-1978, RRID: CVCL_3509 |
| Human melanoma cell: WM-793 |
ATCC |
Cat# CRL-2806, RRID: CVCL_8787 |
| Human urothelial carcinoma cell: BFTC-909 |
DSMZ |
Cat# ACC-367, RRID: CVCL_1084 |
| Human ovarian cancer cell: OVCAR-8 |
NCI-DTP |
Cat# OVCAR-8, RRID: CVCL_1629 |
| Human ovarian cancer cell: OV56 |
ECACC |
Cat# 96020759, RRID: CVCL_2673 |
| Human ovarian cancer cell: SKOV3 |
NICR |
4201PAT-CCTCC01017 |
| Human hepatoma cell: HepG2 |
NICR |
1101HUM-PUMC000035 |
| Normal rat kidney (NRK) epithelial cell line |
NICR |
1101RAT-PUMC000198 |
| L929 mouse C3H/An connective tissue cell line |
NICR |
1101MOU-PUMC000403 |
| HEK-293T |
NICR |
1101HUM-PUMC000010 |
| Experimental models: Organisms/strains |
| Mouse: C57BL/6J |
The Jackson Laboratory |
N/A |
| Mouse: athymic nude mice |
The Jackson Laboratory |
N/A |
| Oligonucleotides |
| ACSL4-sg1, GTGTGTCTGAGGAGATAGCG |
This paper |
N/A |
| ACSL4-sg2, GCATCATCACTCCCTTAGGT |
This paper |
N/A |
| AGPS-sg1, GTACCAATGAGTGCAAAGCG |
This paper |
N/A |
| Recombinant DNA |
| LentiCRISPR V2 plasmid |
(Sanjana et al., 2014) |
Addgene, Cat# 52961 |
| psPAX2 plasmid |
Trono Lab Packaging and Envelope Plasmids (unpublished) |
Addgene, Cat# 12260 |
| pMD2.G plasmid |
Trono Lab Packaging and Envelope Plasmids (unpublished) |
Addgene, Cat# 12259 |
| Software and algorithms |
| ImageStudio software |
LiCor |
https://www.licor.com/bio/image-studio/
|
| Metamorph software |
Molecular Devices, Andor |
|
| ZEN 3.3 blue edition |
Zeiss Microscope |
https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
|
| ImageJ 1.52P |
Fiji |
https://imagej.net/software/fiji/
|
| GraphPad Prism |
GraphPad software Inc |
https://www.graphpad.com/scientific-software/prism/
|
| Nikon NIS Elements software |
Nikon NIS Microscope |
https://www.microscope.healthcare.nikon.com/products/software/nis-elements/viewer
|
| Profinder 10.0 |
Agilent |
N/A |
| Lipid Annotator |
Agilent |
N/A |
| LIPID MAPS |
(Sud et al., 2006) |
https://www.lipidmaps.org
|
| Human Metabolome Database |
(Wishart et al., 2018) |
http://www.hmdb.ca/
|
| Adobe Illustrator |
Adobe |
www.adobe.com
|
| Spearman correlation and cluster analyses |
R-3.3.3 |
|
| Biorender |
|
https://biorender.com/
|
| SciLS Lab v.2021c Pro software |
Bruker Daltonik |
www.bruker.com
|
| Flex control software |
Bruker Daltonik |
www.bruker.com
|
| Flex imaging software |
Bruker Daltonik |
www.bruker.com
|
| Other |
| Accucore C8 column (100 × 2.1 mm, 2.6 μm) |
Thermo Fisher Scientific |
Cat# 17226–102130 |
| RPMI 1640 |
Gibco |
Cat# C11875500BT |
| DMEM |
Gibco |
Cat# C11995500BT |
