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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2021 Dec 9;42(2):175–188. doi: 10.1161/ATVBAHA.121.317197

Figure 2. Adoptive Transfer of BM-Mono or Bone Marrow Mononuclear Lin− Cells.

Figure 2.

Cells from donor mice were injected systemically in recipient mice subjected to hindlimb ischemia the previous day. Monocyte adoptive transfer was performed using BM-Mono from the ischemic leg only (A) or from both legs (B). LDI demonstrates a significant improvement of blood flow recovery in recipient mice who received BM-Mono from the ischemic leg (n=10) (A) and from both legs (n=10) (C); however, no improvement in blood flow recovery was observed in recipient mice who received ischemic-trained Lin− cells (24h trained) compared to recipients with sham Lin− cells (n=9) (E). Remarkably effective arteriogenesis of gracillis collaterals was observed in recipient mice who received BM-Mono from the ischemic leg (B) and from both legs (D). NL: non-ligated, L: ligated. The data were analyzed by 2-way ANOVA, followed by Sidak multiple comparison tests. Errors bars represent SEM (†p<0.01 vs. same timepoint - Sham).