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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2021 Dec 9;42(2):175–188. doi: 10.1161/ATVBAHA.121.317197

Figure 5. Confirmatory qPCR in Sham and 24h trained BM-Mono.

Figure 5.

BM-Mono total RNA was isolated from sham and 24h trained groups (ischemic and contralateral leg) for gene expression quantification. Based on the transcriptomic analysis (Table S1 supplement demonstrated that 15 genes were significantly regulated in ischemic-trained BM-Mono with FDR<0.05), quantification of four genes involved in lipid metabolism, Apoe (A), Dhcr24 (B), SQLE (C), and Lpin1 (D), was performed. Here we demonstrate that Dhcr24, SQLE and Lpin1 were significantly down-regulated in the 24h trained BM-Mono from ischemic leg and three of them in the contralateral leg (median; 95% CI) by Mann-Whitney test.