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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Bone. 2021 Dec 21;156:116302. doi: 10.1016/j.bone.2021.116302

Table 2.

Confocal Raman Spectroscopy after in vitro PTC treatment.

HFD-CTR HFD-PTC p-value
(n = 6) (n = 6)
Carbonate Substitutions (ν1CO3/ν1PO4) 0.881 ± 0.045 0.932 ± 0.064 0.2246
Mineral:Matrix Ratio (ν1PO4/Amide I) 0.971 ± 0.057 1.032 ± 0.152 0.4732
Mineral:Matrix Ratio (ν2PO4/Amide III) 0.948 ± 0.105 0.966 ± 0.049 0.6796
Crystallinity (1/ν1PO4) 0.095 ± 0.004 0.097 ± 0.006 0.4836
CML:CH2-wag (unitless) 1.316 ± 0.227 1.240 ± 0.089 0.4113
PEN:CH2-wag (unitless) 1.285 ± 0.206 1.309 ± 0.089 0.7662
Bound Water (I3220/I2949) 0.390 ± 0.073 0.438 ± 0.161 0.3528

Data are presented as mean ± STD. CML = carboxymethyl-lysine; PEN = pentosidine; CH2 = methylene; PO4 = phosphate; CO3 = carbonate; ν1, ν2 = band mode; fAGEs = fluorescent advanced glycation end-products. Groups represent in vitro treatment with saline (HFD-CTR) or phenacyl thiazolium chloride (HFD-PTC) to femora from ten-week-old C57BL/6J male mice fed a high-fat diet (HFD) for 22 weeks, assessed by paired t-test (two tailed, α = 0.05).