Fig. 2.

Effect of centrifugation during sample preparation on complex I, II, III, and IV substrate linked respiration with postmortem brain samples. Postmortem sample A was used to test the effect of centrifugation during sample preparation on complex I, II, III, and IV substrate linked respiration (OCR) with postmortem brain samples. After sample preparation at the indicated centrifugation forces, 8 μg of protein was loaded per well (n = 3–5 technical replicates per sample preparation). Traces of complexes I (A), II (B), III (C), and IV (D) substrate linked respiration measured as described in Method. (E) Bar graphs comparing calculated complexes I-IV activities. Data are displayed as the mean ± S.E.M.