Fig. 3.

Linear range of complex I, II, III, and IV substrate linked respiration with postmortem brain samples. Postmortem samples A, B and C were used to test the effect of amount of protein on complex I (A), II (B), III (C), and IV (D) substrate linked respiration (OCR) with postmortem brain samples. After sample preparation at 200×g, 2, 4, 8, and 12 μg of protein were loaded per well (n = 3–5 technical replicate wells per sample preparation). Complexes I (A), II (B), III (C), and IV (D) substrate linked respirations were measured as described in Method. Linear regression was used to determine the R² value. Data are displayed as the mean ± S.E.M.