Fig. 4.

Normalization of complex I, II, III, and IV substrate linked respiration to citrate synthase activities. (A) Bar graphs of citrate synthase (CS) activity, lactate dehydrogenase (LDH) activity, as well as the CS/LDH ratio. n = 3 technical replicates per sample. Then we normalized the complex I (B), II (C), III (D), and IV (E) substrate linked respiration (OCR) from Fig. 3 with respective citrate synthase activities. Linear regression was used to determine the R² value. Data are displayed as the mean ± S.E.M.