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Response of postmortem brain lysates to rotenone. Homogenates were pretreated with rotenone for 1 h at the indicated concentrations, followed by measurement of complex I (A,E), II (B,F), III (C,G), and IV (D,H) substrate linked respiration (OCR), with (E-H) and without (A-D) normalization to CS activities. (I) Linear regression was used to determine the R2 value for complex I. Log regression fit was used to calculate IC50. Data are displayed as the mean ± S.E.M.
