| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Isoflurane | Sigma-Aldrich | CAS: 26675-46-7 |
| Buprenorphine | Sigma-Aldrich | CAS: 52485-79-7 |
| Carprofen | Sigma-Aldrich | CAS: 53716-49-7 |
| Ketamine hydrochloride | Sigma-Aldrich | CAS: 1867-66-9 |
| Xylazine hydrochloride | Sigma-Aldrich | CAS: 23076-35-9 |
| Hydrogen peroxide | Sigma-Aldrich | CAS: 7722-84-1 |
| Povidone-iodine | Sigma-Aldrich | CAS: 25655-41-8 |
| Ethanol 70% | Sigma-Aldrich | CAS: 109-56-8 |
| Phosphate buffered saline (PBS) | Sigma-Aldrich | CAS: 7758-11-4 |
| Adhesive luting cement | Parkell | C&B Metabond |
| Light curing glue | Pentron | Flow-it ALC A3 |
| Hair removal cream | Veet | N/A |
| Duratears lubricating eye ointment | Alcon Laboratories | N/A |
| Gelfoam hemostatic sponge | Pfizer | Cat#9032301 |
| Dental acrylic powder | Henry Schein | Cat#09-031 |
| Dental acrylic fluid | Henry Schein | Cat#13-787 |
| Deposited data | ||
| CAD files of head fixation apparatus | This study | https://github.com/leoreh/slutsky_fepsp |
| Spreadsheet for atlas coordinates | This study | https://github.com/leoreh/slutsky_fepsp |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | Strain#000664 |
| Software and algorithms | ||
| pCLAMP data analysis software | Molecular Devices | RRID: SCR_011323; https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite |
| Matlab programming platform | MathWorks | RRID: SCR_001622; https://www.mathworks.com/products/matlab.html |
| ImageJ image processing package | N/A | RRID: SCR_002285; https://imagej.nih.gov/ij/download.html |
| WinWCP | University of Strathclyde | https://github.com/johndempster/WinWCPXE/releases/tag/V5.6.6 |
| Stimulating protocols in winWCP | This study | https://github.com/leoreh/slutsky_fepsp |
| Stimulating protocols in Arduino | This study | https://github.com/leoreh/slutsky_fepsp |
| fEPSP analysis package in MATLAB | This study | https://github.com/leoreh/slutsky_fepsp |
| Other | ||
| Amplifier | A-M systems | Model 1700 |
| Digitizer | Molecular Devices | Digidata 1440A |
| Stimulator | Digitimer | DS3 |
| Micro syringe pump | World Precision Instruments | UMP3 |
| Pump controller | World Precision Instruments | MICRO2T |
| Microliter syringe | Hamilton | Cat#701RN |
| Syringe needle | Hamilton | Cat#7803-03 |
| Magnetic Stirrer | VELP scientifica | Cat#F203A0440 |
| Rotary tool | Dremel | Cat#8300 |
| Cutting wheel for rotary tool | Dremel | Cat#420 |
| Side cutter | NWS | Cat#021F-79-ESD-115 |
| Round nose pliers | NWS | Cat#021B-79-ESD-115 |
| Microscope workshop | Olympus | Cat#SZ51 |
| Soldering station | Weller | Cat#PU 81 |
| Soldering flux | Indalloy | Cat#84003 |
| Soldering wire | Multicore | Cat#362 |
| LED curing light | COXO | Cat#DB-685 |
| Helping hand | Allied electronics | Cat#JA-40 |
| Air duster | Servisol | Cat#235 |
| Sandpaper sheet 60 grit | N/A | N/A |
| Multimeter | Fluke | Cat#77-4 |
| Plasticine | N/A | N/A |
| Adhesive tape | N/A | N/A |
| Slotted screwdriver 2 mm | Wera | Cat#2035 |
| Machined screws | Palboreg Federal LTD | Size#M1x0.2; Standard#DIN 85 |
| Coated stainless steel wire | A-M system | Cat#791400 |
| 3-pin female connector | Sullins Connector Solutions | Cat#S7036-ND |
| 2-pin female connector | Sullins Connector Solutions | Cat#S7035-ND |
| Male connectors | Harwin Inc. | Cat#M20-9990545 |
| Cannula tube | Component supply | Cat#HTX-23T-30 |
| Filler rod | Component supply | Cat#HTX-28R-30 |
| Isoflurane anesthesia vaporizer | Kent Scientific | SomnoSuite |
| DC temperature controller system | FHC | Cat#40-90-8D |
| Small animal stereotaxic instrument | Kopf Instruments | Cat#902 |
| Ear bars | Kopf Instruments | Cat#1921 |
| Mouse head holder | Kopf Instruments | Cat#923-B |
| Micromotor drill | Saeshin | Strong 90 |
| Burr for micro drill | Fine Science Tools | Cat#19007-07 |
| Fine scissors | Fine Science Tools | Cat#14060-11 |
| Skin forceps | Fine Science Tools | Cat#11027-12 |
| Fine forceps | Fine Science Tools | Cat#11231-30 |
| Scalpel handle | Fine Science Tools | Cat#10003-12 |
| Scalpel blades #15 | Fine Science Tools | Cat#10015-00 |
| Hemostat | Fine Science Tools | Cat#13008-12 |
| Bulldog Serrefines | Fine Science Tools | Cat#18050-28 |
| Cotton tip applicators | Cardinal health | Cat#C15055-006 |
| Skin marker | Fine Science Tools | Cat#18000-30 |
| Precision digital weight | American Weigh Scales | Cat#LB-3000 |
| Insulin needles 31g | Novofine | N/A |
| Microscope surgery | Zeiss | Stemi 508 |
| ∗Optical construction post | Thorlabs | Cat#TR3T |
| ∗Optical post | Thorlabs | Cat#TR75/M |
| ∗Rotating clamp | Thorlabs | Cat#SWC/M |
| ∗Post holder | Thorlabs | Cat#PH30/M |
| ∗Running wheel | Bio-serv | Cat#K3251 |
| ∗Head bar | This study | https://github.com/leoreh/slutsky_fepsp |
| ∗Head bar base | This study | https://github.com/leoreh/slutsky_fepsp |
∗
Custom head fixation apparatus.