| Antibodies |
|
|
|
| A full list of antibodies is provided in Table S2
|
N/A |
N/A |
|
| Biological samples |
|
|
|
| The Resource of Archival Breast Tissue (RAHBT) cohort TMAs were compiled at Washington University in St. Louis, all patient information is included in Table S1
|
N/A |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| TBS IHC Wash Buffer with Tween 20 |
Cell Marque |
Cat#935B-09 |
| PBS IHC Wash Buffer with Tween 20 |
Cell Marque |
Cat#934B-09 |
| Target Retrieval Solution, pH 9, (3:1) |
Agilent (Dako) |
Cat#S2375 |
| Avidin/Biotin Blocking Kit |
Biolegend |
Cat#927301 |
| Gelatin (cold water fish skin) |
Sigma-Aldrich |
Cat#G7765–250 |
| Xylene Histological grade |
Sigma-Aldrich |
Cat#534056–500 |
| Glutaraldehyde 8% Aqueous Solution EM Grade |
EMS |
Cat#16020 |
| Normal Donkey serum |
Sigma-Aldrich |
Cat#D9663–10ML |
| Bovine Albumin (BSA) |
Fisher |
Cat#BP1600–100 |
| Centrifugal filters (0.1 μm) |
Millipore |
Cat#UFC30VV00 |
|
| Critical commercial assays |
|
|
|
| MIBItag Conjugation Kit |
IONpath |
Cat#600XXX |
| ImmPRESS UNIVERSAL (Anti-Mouse/Anti-Rabbit) IgG KIT (HRP) |
Vector Laboratories |
Cat#MP-7500–15 |
| ImmPACT DAB (For HRP Substrate) |
Vector Laboratories |
Cat#SK-4105 |
|
| Deposited data |
|
|
|
| All image data, single cell data, and tissue feature data, is located in a public Mendeley data repository: https://data.mendeley.com/datasets/d87vg86zd8
|
Mendeley Data |
https://data.mendeley.com/datasets/d87vg86zd8
|
|
| Software and algorithms |
|
|
|
| Data analysis was done using MATLAB 2016 |
Mathworks |
N/A |
| Data analysis was done using R 3.6.1 |
R |
N/A |
| Data analysis was done using Python |
Python |
N/A |
| Analysis code for MATLAB, R, and Python is available at the Mendeley data and code repository: https://data.mendeley.com/datasets/d87vg86zd8
|
Mendeley Data |
https://data.mendeley.com/datasets/d87vg86zd8
|
