FIGURE 7.

Surfactant protein C (SFTPC) C-terminal cleavage is inhibited by Z-VLL-CHO. a) HeLa cells stably expressing green fluorescent protein (GFP)-SFTPC-wild type (WT) or -I73T were treated with 5 μM Z-VLL-CHO for 16 h and lysates immunoblotted for NproSFTPC. Cells treated with Z-VLL-CHO develop an excess of early C-terminal processing intermediates, increased overall SFTPC (as measured by GFP) and redistribution of SFTPC to the plasma membrane, as seen by b) confocal microscopy and c) flow cytometry. Scale bar=10 μm.