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PCR analysis of TW fluid samples using VP and 16S primers
| Primers | No. (%) of positive samples with:
|
|
|---|---|---|
| No anticoagulant (n = 53) | EDTA (n = 45) | |
| VP positive only | 5 (9.4) | 5 (11.1) |
| 16S positive only | 12 (22.6) | 10 (22.2) |
| VP and 16S positive | 12 (22.6) | 7 (15.5) |
| VP and 16S negative | 22 (41.5) | 22 (48.9) |
| Unreadablea | 2 (3.8) | 1 (2.2) |
“Unreadable” samples produced a heavy smear on the ethidium bromide-stained agarose gel.
