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. 2022 Jan 13;12:802584. doi: 10.3389/fphys.2021.802584

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Copyright © 2022 Gong, Li, Li, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HPLC profiles of PBCHO (3-phenoxybenzaldehyde) metabolism mediated by expressed CYP/CPR microsomal proteins. Black arrows indicate the substrate PBCHO, or the reduced conversion: PBOH. Red arrows indicate the oxidation product PBCOOH. The in vitro reactions contained 10 μM PBCHO, 1 mg of CYP/CPR microsomes, 0.25 mM MgCl₂, and 1 mM NADPH for a total reaction volume of 700 μL; NADPH was omitted in the Control reaction. Each CYP/CPR protein with NADPH has a control reaction with the same microsome without NADPH. However, only the chromatogram of control reaction CYP6BZ2 without NADPH was shown in this figure.