FIG. 1.

(A) Partial VP1 amino acid alignment for three E30 reference strains and several related nonpoliovirus EVs. The E30 strains tested included Bastianni (E30B; E30 prototype strain, New York, 1958), Frater (E30F; Scotland, 1959), and Giles (E30G; Minnesota, 1960) (24). Sites that are identical in sequence to that of E30 Bastianni are indicated in the alignment by a period. Hyphens indicate gaps inserted to create the alignment. Motifs targeted by RT-PCR primers are boxed. CA, coxsackievirus type A; CB, coxsackievirus type B. (B) Sequences of degenerate primers 120S, 157S, and 47A. The E30 amino acids used to design the primers are shown above the primer sequences.