RT-PCR amplification of E30 isolates and non-E30 echovirus prototype strains with primer pair 157S-47A. The position of the 158-bp E30-specific product is indicated by an asterisk. Lanes N, negative control; lanes C, positive control (E30 Frater); lanes M, molecular weight marker V (Boehringer Mannheim). (A) Lanes 1 to 12, E30 isolates AUS56-3999, MI67-0258, ID76-8267, COL95-6669, NV76-8213, NC81-3092, CA67-T673125, AUS73-120112, TRT83-4794, PQ88-8331, DEU96-2308, and SD98-2730, respectively. (B) Lanes 1 to 12, E6, E7, E8, E9, E12, E13, E14, E16, E21, E25, E29, and E32, respectively.