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. Author manuscript; available in PMC: 2022 Jan 27.
Published in final edited form as: Nat Protoc. 2021 Jan 13;16(2):1193–1218. doi: 10.1038/s41596-020-00454-5

Fig. 4|.

Fig. 4|

Agarose gel and tapestation results. a, Agarose gels visualized after sucrose gradient fractionation of genomic DNA from human cell lines (as depicted here by HCT116 cells). Only the fractions containing fragments that are not longer than 200 bp are pooled together and concentrated. The first 8 fractions (shown within the red box) typically contain the fragments of the desired sizes. b, Library preparation by PCR amplification as indicated by the smear (*) above the primer-dimer band that is present at around 120 bp. c, Tapestation analysis of size-distribution and quality of library. Library size distribution is indicated by the smear (*) above the primer-dimer band. The green and pink bands mark the upper and lower markers respectively.