Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells

. Author manuscript; available in PMC: 2022 Jan 27.

Published in final edited form as: Nat Protoc. 2021 Jan 13;16(2):1193–1218. doi: 10.1038/s41596-020-00454-5

Cycle	Denature	Extend	Anneal	Hold
1	95 °C 2 min
2–14	98 °C 30 s	62.5 °C 30 s	72 °C 30 s
15			72 °C 5 min
16				4 °C