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. Author manuscript; available in PMC: 2022 Jan 27.
Published in final edited form as: Nat Protoc. 2021 Jan 13;16(2):1193–1218. doi: 10.1038/s41596-020-00454-5
Reagent Volume (μl) Final concentration
DNA sample (Step 48) 320–380
Click-it reaction buffer 10× or Tris-HCl pH 8 100mM 50 10 mM
CuSO4 100mM 10 2 mM
Biotin TEG Azide 100mM 10 2 mM
Ascorbate Na 100mM 50 10 mM
Milli-Q Water to 500
Total 500