| Reagent | Volume (μL) |
|---|---|
| Inserts (resuspended DNA in water; Step 68) | 29 |
| Purified 5’ adapter pair [2 μg/μl] | 1.5 |
| Purified 3’ adapter pair [2 μg/μl] | 1.5 |
| T4 Ligase Buffer | 4 |
| T4 DNA Ligase | 4 |
| Total | 40 |
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