KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-SORL1 | Cell Signaling Technology | Cat#79322S; RRID: AB_2799927 |
| Rabbit polyclonal anti-VPS26b | Novus Biologicals | Cat#NBP1-92575; RRID: AB_11020053 |
| Rabbit polyclonal anti-VPS26a | Abcam | Cat#ab23892; RRID: AB_2215043 |
| Rabbit polyclonal anti-VPS26a | Bonifacino Lab; Rojas et al. (2008) | N/A |
| Rabbit polyclonal anti-Neuro-tubulin | Abcam | Cat#ab18207; RRID: AB_444319 |
| Rabbit polyclonal anti-VPS35 | Bethyl laboratories | Cat#A304-727A; RRID: AB_2620922 |
| Rabbit polyclonal anti-GluA1 C-terminus | MilliporeSigma | Cat#AB1504; RRID: AB_2113602 |
| Rabbit polyclonal anti-mGluR5 | Alomones lab | Cat#AGC-007; RRID: AB_2039991 |
| Rabbit polyclonal anti-Syntaxin13 | Synaptic Systems | Cat#110 133; RRID: AB_2198225 |
| Rabbit polyclonal anti-Rab11 | Thermo Fisher Scientific | Cat#71–5300; RRID: AB_2533987 |
| Rabbit polyclonal anti-Syntaxin13 | Synaptic Systems | Cat#110 132; RRID: AB_67552 |
| Rabbit polyclonal anti-MAP2 | MilliporeSigma | Cat#AB5622, RRID: AB_91939 |
| Goat polyclonal anti-VPS35 | Abcam | Cat#ab10099; RRID: AB_296841 |
| Goat polyclonal anti-VPS29 | MilliporeSigma | Cat#SAB2501105; RRID: AB_10602838 |
| Goat polyclonal anti-EEA1 | Santa Cruz Biotechnology, Inc. | Cat#sc-6415; RRID: AB_2096822 |
| Mouse monoclonal anti-β-actin | Novus Biologicals | Cat#NB600-535; RRID: AB_2222878 |
| Mouse monoclonal anti-α-tubulin | MilliporeSigma | Cat#T6074; RRID: AB_477582 |
| Mouse monoclonal anti-VPS35 | Abcam | Cat#ab57632; RRID: AB_946126 |
| Mouse monoclonal anti-VPS26a | Novus Biologicals | Cat#NBP2-36754 |
| Mouse monoclonal anti-Golgin97 | Thermo Fisher Scientific | Cat#A-21270; RRID: AB_221447 |
| Mouse monoclonal anti-Rab7 | Santa Cruz Biotechnology, Inc. | Cat#sc-376362; RRID: AB_10987863 |
| Mouse monoclonal anti-GluA1 N-terminus | MilliporeSigma | Cat#MAB2263; RRID: AB_11212678 |
| Mouse monoclonal anti-Neuro-tubulin | BioLegend, Inc. | Cat#801202, RRID: AB_10063408 |
| Mouse monoclonal anti-Rab5 | Santa Cruz Biotechnology, Inc. | Cat#sc-46692, RRID: AB_628191 |
| Chicken polyclonal anti-GFAP | Abcam | Cat#ab4674, RRID: AB_304558 |
| Chicken polyclonal anti-MAP2 | Abcam | Cat#ab5392; RRID: AB_2138153 |
| IRDye® 800CW Donkey anti-Rabbit IgG Secondary Antibody | LI-COR Biosciences | Cat#926–32213; RRID: AB_621848 |
| IRDye® 680RD Donkey anti-Mouse IgG Secondary Antibody | LI-COR Biosciences | Cat#926–68072; RRID: AB_10953628 |
| IRDye® 680RD Donkey anti-Goat IgG Secondary Antibody | LI-COR Biosciences | Cat#926–68074; RRID: AB_10956736 |
| IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody | LI-COR Biosciences | Cat#926–32212; RRID: AB_621847 |
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#21206; RRID: AB_2535792 |
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 | Thermo Fisher Scientific | Cat# A-31572; RRID: AB_162543 |
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-31573; RRID: AB_2536183 |
| Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21202; RRID: AB_141607 |
| Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 | Thermo Fisher Scientific | Cat#31570; RRID: AB_2536180 |
| Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#31571; RRID: AB_162542 |
| Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#11055; RRID: AB_2534102 |
| Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 | Thermo Fisher Scientific | Cat#21432; RRID: AB_2535853 |
| Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#21447; RRID: AB_141844 |
| Chemicals, peptides, and recombinant proteins | ||
| Transferrin From Human Serum, Alexa Fluor™ 555 Conjugate | Thermo Fisher Scientific | Cat#T35352 |
| (−)-Bicuculline methiodide | Tocris | Cat#2503; CAS Number: 40709-69-1 |
| EZ-Link™ Sulfo-NHS-LC-Biotin | Thermo Fisher Scientific | Cat#21335; CAS Number: 127062-22-0 |
| Picrotoxin | MilliporeSigma | Cat# P1675-1G; CAS Number: 124-87-8 |
| Glycine | MilliporeSigma | Cat# G7126-100G; CAS Number: 56-40-6 |
| 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f] quinoxaline-2,3-dione | MilliporeSigma | Cat#N183-5MG; CAS Number: 118876-58-7 |
| (2R)-amino-5-phosphonovaleric acid | MilliporeSigma | Cat#A5282; CAS Number: 76326-31-3 |
| Atropine | MilliporeSigma | Cat#A0132; CAS Number: 51-55-8 |
| VPS26A Recombinant Protein Antigen | Novus Biologicals | Cat# NBP2-36754PEP |
| Critical commercial assays | ||
| Pierce™ BCA Protein Assay Kit | Thermo Fisher Scientific | Cat#23227 |
| Mouse Hemoglobin ELISA Kit | Abcam | Cat#ab157715 |
| Simoa™ Mouse Tau Discovery Kit | Quanterix | Cat#102209 |
| Amyloid beta 42 Mouse ELISA Kit | Thermo Fisher Scientific | Cat#KMB3441 |
| Amyloid beta 40 Mouse ELISA Kit | Thermo Fisher Scientific | Cat#KMB3481 |
| Deposited data | ||
| https://doi.org/10.17632/jggdkrpxts.1 | N/A | |
| Experimental models: Organisms/strains | ||
| Mouse: Vps26b HET/KO mice | Kim et al. (2008) | N/A |
| Mouse: Vps26a HET mice | Muhammad et al. (2008) | N/A |
| Mouse: Vps26aflox/flox mice | Mecozzi et al. (2014) | N/A |
| Mouse: Vps35flox/flox mice | Simoes et al. (2020) | N/A |
| Recombinant DNA | ||
| Second generation lentiviral packaging plasmids VSVg and Δ8.9 | Peter Scheiffele (University of Basel) | N/A |
| EGFP-tagged active, full-length Cre recombinase and catalytically inactive, truncated Cre FUGW lentiviral constructs | Thomas Sudhof (Stanford University, California) | N/A |
| p-Lenti-C-mGFP-P2A-Puro | OriGene Technologies, Inc. | Cat#PS100093 |
| p-Lenti-C-mGFP VPS26b | OriGene Technologies, Inc. | Cat#MR204988L4 |
| Lentiviral Packaging Kits | OriGene Technologies, Inc. | Cat#TR30037 |
| AAV9-CAG-VPS26b-2A-eGFP-WPRE vector | Vector Biolabs | N/A |
| AAV9-CAG-eGFP-WPRE | Vector Biolabs | N/A |
| Software and algorithms | ||
| EthoVision XT 7.0 | Noldus Information Technology | https://www.noldus.com/ethovision-xt |
| SigmaPlot 14.5 | Systat Software Inc. | https://systatsoftware.com/products/ |
| ImageJ 1.53e | NIH | https://imagej.nih.gov/ij/download.html |
| Image Studio Lite 5.0 | LI-COR Biosciences | https://www.licor.com/bio/image-studio-lite/ |
| MATLAB R2021a | The MathWorks, Inc. | https://www.mathworks.com/products/matlab.html |
| GraphPad Prism 9.00 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| SPSS 24.0 | IBM | https://www.ibm.com/products/spss-statistics |
| ANY-maze 6.3 | Stoelting Co. | https://stoeltingco.com/Neuroscience/ANY-maze |
| Other | ||
| Butterfly needle to collect CSF | Terumo Medical Products | Cat#SV*27EL |
| Neurobasal™-A Medium | Thermo Fisher Scientific | Cat#10888022 |
| B-27™ Supplement (50X), serum free | Thermo Fisher Scientific | Cat#17504044 |