Figure 1.

Identification and detection of tertiary lymphoid structures. (A–D). H&E, early tertiary lymphoid structures for lymphocyte aggregation, (B) primary lymphoid structures; (C) secondary lymphoid structures, germinal centers positive (GC-positive), (D) secondary lymphoid structures with GC. (D–H). the immunohistochemistry of. CD20⁺ B cells, CD21⁺ follicular helper T cells, and CD23⁺ germinal Centre scenters; (I–L) the co-stained immunofluorescence of CD20⁺, CD21⁺, and CD23⁺; (L) is merged by CD20⁺, CD21⁺, and CD23⁺’s merged. (M) The distribution of TLS density. (N–P) The relationship between age, gender and hepatitis. “ns” means no significance.