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Fluorescence titration binding study of oligo-1: d(CGCGAATTCGCG)2 and oligo-2: d(CGATGTACATCG)2 with ligands (11a, 12a, and 12b). 1–20 μL of oligos (stock 0.1 mM) were added to a solution of ligand (1 mL of a 1 mM solution), and emission scanning was carried out from 360 to 680 at 2 nm/s speed; 5 nm slit width; T = 20 °C in a buffer containing 20 mM sodium cacodylate (pH 7.2) and 100 mM Na.
