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Purification and expression of TRPV1. (A) Large-scale purification of TRPV1/pcDNA3.1+/C-(K)DYK plasmid DNA. Lane 1: 300 ng (parental plasmid DNA); lane 2: 200 ng newly purified plasmid; and lane 3: 400 ng newly purified plasmid. (B) TRPV1 protein expression in HEK-293 cells detected by Western blot. Lane 1: protein ladder; lane 2: HEK-293 cell alone; lane 3: 18 h post-transfection; and lane 4: 23 h post-transfection.
