Skip to main content
. 2022 Jan 13;8:807301. doi: 10.3389/fvets.2021.807301

Figure 4.

Figure 4

RES improved the negative effect on mitochondrial respiration by DON in vitro. (A) Schematic and (B) oxygen consumption rate (OCR) assessed by extracellular flux analysis. OCR was measured under basal conditions followed by the sequential addition of oligomycin (0.5 μM), FCCP (1 μM), rotenone (1 μM), or antimycin A (1 μM). Each data point represents an OCR measurement. (C) Individual parameters for basal respiration, proton leak, maximal respiration, spare respiratory capacity, nonmitochondrial respiration, and ATP production were determined. Cells were treated with 0 (NC) or 0.5μmol/L or 1μmol/L DON and 0 or 15μM RES, respectively. Data were expressed as means ± SEM of at least three independent experiments. (A–C) Values with different letters are significantly different (P < 0.05).