TABLE 1.

primers used.

Genes	Primer sequence 5′-3′	Amplicon length (bp)	ENSEMBL gene ID
Housekeeping gene
HPRT	Fw TgA​CCT​TgA​TTT​ATT​TgC​ATA​CC	101	ENSG00000165704
	Rv CGA​gCA​AgA​CgT​TCA​gTC​CT
Β2M	Fw CgACggCCgCTgTCA	100	ENSG00000160255
	Rv TgT​TgT​TTT​CAg​CCA​gCT​TgT​g
Osteogenesis
RUNX2	Fw CCA​gAA​ggC​ACA​gAC​AgA​AgC​T	156	ENSG00000124813
	Rv Agg​AAT​gCg​CCC​TAA​ATC​ACT
Collagen I	Fw TCC​AAC​gAg​ATC​gAg​ATC​C	190	ENSG00000108821
	Rv AAgCCgAATTCCTggTCT
Osteonectin	Fw TAC​ATC​ggg​CCT​TgC​AAA​TAC	100	ENSG00000231061
	Rv Agg​gTg​ACC​Agg​ACg​TTC​TTg
Osteoclastogenesis		67	ENSG00000141655
RANK	Fw CCT​ggA​CCA​ACT​gTA​CCT​TCC​T
	Rv ACC​gCA​TCg​gAT​TTC​TCT​gT	60	ENSG00000120659
RANKL	Fw CAT​CCC​ATC​Tgg​TTC​CCA​TAA
	Rv gCCCAACCCCgATCATg	100	ENSG00000164761
OPG	Fw CTgCgCgCTCgTgTTTC
	Rv ACA​gCT​gAT​gAg​Agg​TTT​CTT​CgT	100	ENSG00000184371
M-CSF	Fw CCg​Agg​Agg​TgT​Cgg​AgT​AC
	Rv AAT​TTg​gCA​CgA​ggT​CTC​CAT
DC-STAMP	Fw ATT​TTC​TCA​gTg​AgC​AAg​CAg​TTT​C	101	ENSG00000164935
	Rv gAA​TCA​Tgg​ATA​ATA​TCT​TgA​gTT​CCT​T
TRAcP	Fw CAC​AAT​CTg​CAg​TAC​CTg​CAA​ggA​T	128	ENSG00000102575
	Rv CCC​ATA​GTg​gAA​gCg​CAg​ATA
CD14	Fw ggT​TCg​gAA​gAC​TTA​TCg​ACC​AT	109	ENSG00000170458
	Rv TCA​TCg​TCC​AgC​TCA​CAA​ggT
Cathepsin K	Fw CCA​TAT​gTg​ggA​CAg​gAA​gAg​AgT​T	149	ENSG00000143387
	Rv TgC​ATC​AAT​ggC​CAC​AgA​gA
Nfatc1	Fw AgC​AgA​gCA​Cgg​ACA​gCT​ATC	143	ENSG00000131196
	Rv ggT​CAg​TTT​TCg​CTT​CCA​TCT​C
Cox1	5′ AgT​ACA​gCT​ACg​AgC​AgT​T 3′	100	ENSG00000229899
	5′ gAg​ACT​CCC​TgA​TgA​CAT​CC 3′
Cox2	5′ gCA​TTC​TTT​gCC​CAg​CAC​TT 3′	100	ENSG00000267084
	5′ AgA​CCA​ggC​ACC​AgA​CCA​AAg​A 3′