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Marrow MSCs (CFU-F) and age. Bone marrow was obtained, dispersed, placed on a Percoll gradient and the light cell fraction seeded into culture [7]. After 7–10 days, colonies can be visualized as first described by Friedenstein and colleagues [8,9]. The CFU-F assay is an incomplete and crude estimate for the titre of MSCs in each marrow sample. Clearly, the number of MSCs in marrow decreases with age [8]
