TABLE 1.
Nucleotide sequences of primers used to detect the cagA genea
Primer | Sequence (5′ to 3′) | Location |
---|---|---|
A1 | GATAACAGGCAAGCTTTTGAGGGA | 157–181 |
A2 | CCATGAATTTTTGATCCGTTC | 550–527 |
A3 | ATGGGGAGTCATGATGGCATAGAACC | 910–935 |
A4 | ATTAGGCAAATTAAAGACAGCCACC | 1626–1602 |
The indicated primers were used in PCRs consisting of 40 cycles as follows: 1 min at 94°C, 1 min at 58°C, and 1 min at 72°C.