Fig. 6.

Exo-155-5p-treated macrophages activate cytotoxic T cells. A-B. Macrophages were pretreated with Exo-con, Exo-NAC, Exo-miR-con or Exo-miR-155 at 100 μg/ml for 48 h followed by co-cultured with activated T cells for another 48 h. Flow cytometry was performed to analyze CD8⁺ T cells proportion (A) and CD3⁺ T cell apoptosis (B). C. CD3⁺ T cells were sorted from the co-culture systems using FACS. Total RNA was extracted and the mRNA levels of chemokines were measured by RT-qPCR. D. Macrophages were transfected with PD-L1 or vector for 48 h before the Exo-miR-155 treatment followed by the co-culture with T cells for another 48 h. CD4⁺ and CD8⁺ T cells proportions were determined by flow cytometry. Data are representative of 3 independent experiments. Data = Mean ± SD. *p < 0.05, **p < 0.01. Abbreviations: Exo-con: Exo-con-treated macrophages + T cells; Exo-NAC: Exo-NAC-treated macrophages + T cells; Exo-miR-con: Exo-miR-con-treated macrophages + T cells; Exo-miR-155: Exo-miR-155-treated macrophages + T cells; Exo-miR-155 + PD-L1: macrophages overexpressed PD-L1 with Exo-miR-155 treatment + T cells