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. 2022 Jan 27;22:5. doi: 10.1186/s12896-022-00735-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Phylogenetic analyses of DSX homologues. A–D Multiple sequence alignment of DSX protein isoforms in Lepidoptera. The sequences are divided into common male and female region (A), F1 and F2 female-specific region (B and C, respectively) and male-specific region (D). The DNA binding (OD1) and oligomerisation (OD2) domains are boxed and S. frugiperda DSX protein is highlighted in red. E. Phylogenetic tree based on the combined OD1 and OD2 DSX protein sequences in Lepidoptera and D. melanogaster. Bootstrap support is indicated above each branch. F. Schematic representation of Sfdsx gene splicing. The minimal sex-specific splicing module, spanning exons 2 to 5 is highlighted. Differential splicing is indicated above (F1 and F2 isoforms) and below (M splice form) the gene