Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 27;22:5. doi: 10.1186/s12896-022-00735-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — OX5382 construct schematics and splicing. A Linear construct map for OX5382. The position of the conditional female-specific self-limiting gene and that of the fluorescent marker is indicated. B Predicted splicing pattern of OX5382. The mRNA is predicted to generate three major sex-specific splice forms: F1, F2 and M. The F1 and F2 female-specific splice forms are in-frame with tTAV, while the male-specific M isoform is not. Translation start sites are indicated by an arrow; in-frame stop codons are indicated by red octagons. The ubiquitin cleavage site in the translated F1 and F2 DSX proteins is indicated by scissors. C Representative mRNA splicing analysis on OX5382 G₁ adults reared on tetracycline. Both endogenous Sfdsx (top gel) and construct-derived OX5382 Sfdsx show the predicted sex-specific splicing pattern. Non-injected wild-type individuals were included in the assay as a negative control for the construct-specific splicing PCR. NoRT: non-reverse transcribed control; NTC: no template control