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. 2022 Jan 13;12:815946. doi: 10.3389/fpls.2021.815946

TABLE 1.

Summary of T0 plant characterization.

Target site Varieties Genome editing tool No. of T0 plants tested Mutated T0 lines: number, ratio (%) Homozygous T0 lines: number, ratio (%) Bi-allelic T0 lines: number, ratio (%) Heterozygous T0 lines: number, ratio (%)
FMBP sgRNA2 Yugu1 SpCas9 7 7, 100% 2, 28.6% 5, 71.4% N.D
Dof4 sgRNA1 Yugu1 SpCas9 7 7, 100% 6, 85.7% 1, 14.3% N.D
BADH2 sgRNA2 Yugu1 SpCas9 6 5 83.3% 2, 33.3% 3, 50% N.D
GBSS1 sgRNA2 Yugu1 SpCas9 3 3, 100% 1, 33.3% 2, 66.7% N.D
Dof4 sgRNA1 xiaomi SpCas9 2 2, 100% 1, 50% 1, 50% N.D
ALS sgRNA xiaomi CBE 2 1, 50% N.D N.D N.D
ACC sgRNA xiaomi ABE 13 4, 30.8% 2, 15.4% N.D 2, 15.4%