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. 2022 Jan 13;12:812947. doi: 10.3389/fphys.2021.812947

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Copyright © 2022 Melo Ferreira, Freije and Eadon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deconvolution techniques in spatial transcriptomics. (A) H + E image of a human nephrectomy sample, (B) NPHS2 (podocin gene) expression localizes over glomeruli. (C) Seurat deconvolution in the same nephrectomy field. (D) Magnified field of H + E image. (E) Cell type identity legend. (F) Zoomed image of the Seurat deconvolution. (G) SPOTlight deconvolution. (H) Robust cell type decomposition deconvolution. (I) BayesSpace Deconvolution. (J) Zoomed field of SPOTlight deconvolution. (K) Zoomed field of RCTD. (L) Zoomed field of BayesSpace. Each spot is 55 μm in diameter.