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. 2022 Jan 13;12:812947. doi: 10.3389/fphys.2021.812947

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Copyright © 2022 Melo Ferreira, Freije and Eadon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Normalization and batch correction of spatial transcriptomic samples. (A) Uncorrected GAPDH expression across samples. (B) Normalized GAPDH expression with SCTransform. (C) Normalization and batch correction were performed by adding a batch as a variable in SCTransform. (D) Uncorrected expression of ACTB. (E) Normalized expression of ACTB. (F) Batch-corrected expression of ACTB. Sample A is batch 1, samples B–E are batch 2, and samples F–I are batch 3.