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. 2022 Jan 13;9:818053. doi: 10.3389/fbioe.2021.818053

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Copyright © 2022 Suvarli, Wenger, Serra, Perner-Nochta, Hubbuch and Wörner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Graphic representation of the activity assay evaluation method. ONP calibration curves were calculated from known ONP samples (A) to transform the obtained absorbance data of the activity assays (B) to ONP concentration profiles over time (C). The maximum activity of a sample (a _max) corresponds to the maximum slope of the respective curve and was determined using a 12 min rolling-window analysis. The obtained values of the maximum activity were presented using bar plots (D). Depicted here are generic samples.