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. 2018 Jan 4;32(2):576–587. doi: 10.1096/fj.201700393RR

Figure 2.

Figure 2

SGK3 knockdown results in podocyte injury in vitro. MPCs were infected with SGK3‐eGFP shRNA. A). Immunofluorescence revealed the effect of transfection with SGK3 shRNA. B) The SGK3 mRNA expression level was determined by real‐time PCR. C) Top: immunoblot analysis for SGK3 expression; GAPDH was used for normalization. Bottom: quantification of SGK3 by densitometry. D) Podocyte viability was determined by MTT. E) The mRNA expression levels of podocin and desmin were detected using real‐time PCR. F) Top: immunoblot analysis for desmin, podocin, and CD2AP expression. Bottom: quantification of desmin, podocin, and CD2AP by densitometry. OD, optical density. Data are means ± se of 3 independent experiments; 3 wells were repeated for each sample. *P < 0.05 vs. control group, **P < 0.01 vs. control group.