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. 2018 Jan 4;32(2):576–587. doi: 10.1096/fj.201700393RR

Figure 3.

Figure 3

SGK3 activation mostly reversed PAN‐induced podocyte injury. The SGK3‐S486D plasmid was transiently transfected into cultured MPCs, and then the podocytes were exposed for 24 h to 0 and 25 μg/ml PAN. A) Top: immunoblot analysis for SGK3 expression; GAPDH was used for normalization. Bottom: quantification of SGK3 by densitometry. B) Podocyte viability was determined by MTT. C) Top: immunoblot analysis for desmin and podocin expression. Bottom: quantification of desmin and podocin by densitometry. D) Top: immunoblot analysis for CD2AP expression. Bottom: quantification of CD2AP by densitometry. OD, optical density. Data are means ± se of in 3 independent experiments. *P < 0.05 vs. PAN–/S486D– group, **P < 0.01 vs. PAN‐/S486D– group, ## P < 0.01 vs. PAN+/S486D– group.