FIGURE 8.

USP25 aggravates acute pancreatitis and acute pancreatitis-related multiple organ injury by destroying tight junctions through activating STAT3 pathway in vitro and in vivo. Stattic was used to interfere with STAT3 phosphorylation in AR42J, MPAC cells and mice (n = 8 per group), then cerulein was used to induce AP. Colivelin (STAT3 agonist) was used to activate STAT3 in AR42J and MPAC cells, and then cerulein was used to induce AP in cells (A–B) Western blotting was performed to measure the protein levels of USP25, STAT3, p-STAT3^Y705, and ZO-1 in AR42J, MPAC cells and mice pancreases after stattic treatment. (C) Immunofluorescence was performed to measure the protein level of ZO-1 in mice pancreases of eight groups. (D) Western blotting was performed to measure the protein levels of USP25, STAT3, p-STAT3^Y705, and ZO-1 in AR42J, MPAC cells after colivelin treatmenet.